Application
Western blot analysis of extracts of various cells, using CTCF antibody.Application
Chromatin Immunoprecipitation analysis extracts of HCT116 cell line, using CTCF rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Application
Immunohistochemistry of paraffin-embedded human lung cancer using CTCF antibody at dilution of 1:100 (40x lens).Application
Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody at dilution of 1:100 (40x lens).| Product Name | CTCF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CTCFThis gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Immunogen | Recombinant protein of human CTCF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWBChIP |
| WB 1:500 - 1:2000, IHC 1:100 - 1:200, ChIP 1:20 - 1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCCTC-binding factor (zinc finger protein) |
|---|---|
| Target | CTCF |
| Molecular Weight(MW) | 83 kDa (note) |
Application
Western blot analysis of extracts of various cells, using CTCF antibody.
Application
Chromatin Immunoprecipitation analysis extracts of HCT116 cell line, using CTCF rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application
Immunohistochemistry of paraffin-embedded human lung cancer using CTCF antibody at dilution of 1:100 (40x lens).
Application
Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody at dilution of 1:100 (40x lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:100 - 1:200, ChIP 1:20 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
