Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CTGF antibody at 1:500 dilution.Application
Sample (50 µg of whole cell lysate) A: mouse heart 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CTGF antibody detects CTGF protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human CTGF (+). 10% SDS-PAGE CTGF antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (1 µg of whole cell lysate) A: Rat bladder 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HUVEC 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CTGF antibody detects CTGF protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CTGF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CTGF (connective tissue growth factor)The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CTGF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Sheep, Chimpanzee, BovineXenopus laevis (85%), Pig (96%), Chicken (94%), Sheep (96%), Chimpanzee (99%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCN2 antibody, HCS24 antibody, IGFBP8 antibody, MGC102839 antibody, CTGF antibody, connective tissue growth factor antibody, IGFBP-8 antibody, insulin-like growth factor-binding protein 8 antibody, hypertrophic chondrocyte-specific protein 24 antibody, IBP |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CTGF antibody at 1:500 dilution.
Application
Sample (50 µg of whole cell lysate) A: mouse heart 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CTGF antibody detects CTGF protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human CTGF (+). 10% SDS-PAGE CTGF antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (1 µg of whole cell lysate) A: Rat bladder 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HUVEC 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CTGF antibody detects CTGF protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
