Application
Western blot analysis of extracts of various cell lines, using CTH antibody.WB
(C) Western blot analysis (left) and quantitative analysis (right) of CBS, CSE and MPST protein expression in lung cancer cell lines (A549 and 95D) and normal lung cell line (BEAS-2B).WB
(B) Western blot analysis of CBS and CSE expressed in A549 cells after transfection.(H) Western blot analysis of CBS and CSE expressed in BEAS-2B cells after infection.WB
(C) Western blot analysis and qRT-PCR analysis of CBS, CSE and MPST expression after 200 μM CoCl2 treatment for 24 h.(F) Western blot and qRT-PCR analysis of H2S-producing enzymes and HIF-1α expression after transfection with pcDNA3.0/mut HIF-1α plasmid or empty vector control.(H) The expression levels of CBS, CSE, MPST and HIF-1α in protein and mRNA were detected using western blot and qRT-PCR analysis after transfection with si-HIF-1α or si-negative control (si-NC), respectively.WB
(C) Western blot analysis of CBS, CSE, and MPST protein expression levels.| Product Name | CTH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CTHThis gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010] |
| Immunogen | Recombinant protein of human CTH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cystathionine gamma-lyase |
|---|---|
| Target | CTH |
Application
Western blot analysis of extracts of various cell lines, using CTH antibody.
WB
(C) Western blot analysis (left) and quantitative analysis (right) of CBS, CSE and MPST protein expression in lung cancer cell lines (A549 and 95D) and normal lung cell line (BEAS-2B).
WB
(B) Western blot analysis of CBS and CSE expressed in A549 cells after transfection.(H) Western blot analysis of CBS and CSE expressed in BEAS-2B cells after infection.
WB
(C) Western blot analysis and qRT-PCR analysis of CBS, CSE and MPST expression after 200 μM CoCl2 treatment for 24 h.(F) Western blot and qRT-PCR analysis of H2S-producing enzymes and HIF-1α expression after transfection with pcDNA3.0/mut HIF-1α plasmid or empty vector control.(H) The expression levels of CBS, CSE, MPST and HIF-1α in protein and mRNA were detected using western blot and qRT-PCR analysis after transfection with si-HIF-1α or si-negative control (si-NC), respectively.
WB
(C) Western blot analysis of CBS, CSE, and MPST protein expression levels.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
