| Product Name | Cullin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cullin 1CUL 1 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2, both of which, together with cdc 53 (known as CUL A), as three subunits form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The CUL 1/p19 skp1/p45 skp2complex is thought to play a role in the ubiquitination of G1 regulatory proteins. |
| Immunogen | Synthetic peptide: C-DGEKDTYSYLA, corresponding to amino acids 742-752 of Human Cullin 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| Immunoprecipitation (native): use at a concentration of 10μg / mg (protein A). Western Blotting: use at a concentration of 5 - 10μg / ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 5 - 10μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 86 kDa, which corresponds to the predicted molecular weight of Cullin 1. No special pretreatment is required for immuno histochemical staining of formalin fixed tissues.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 8454 603134 Cullin 1 Q13616 CUL1 Cullin1 |
|---|---|
| Target | Cullin 1 |
| Positive Control | HeLa cells and Tonsil |
|---|---|
| Application Notes | Immunoprecipitation (native): use at a concentration of 10μg / mg (protein A). Western Blotting: use at a concentration of 5 - 10μg / ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 5 - 10μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 86 kDa, which corresponds to the predicted molecular weight of Cullin 1. No special pretreatment is required for immuno histochemical staining of formalin fixed tissues.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
