Rabbit polyclonal to Cullin 2 predilutedCullin 2 is a member of the family of human Cullin genes (CUL1, 2, 3, 4a, 4b and 5) homologous to the S. cerevisiae cdc53 gene. CUL 1 forms a complex with human p19skp1 and F box protein p45skp2, together with cdc53, as three subunits, form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL 1, CUL 2 does not associate with p19skp1 and p45skp2. CUL 2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL.
Immunogen
Synthetic peptide (Human) (C terminal).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-P
IHC-P: Ready diluted. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.:
Human Tonsil stained with anti-Cullin-2 (CUL-2) antibody
Positive Control
Raji cells and tonsil cells
Application Notes
IHC-P: Ready diluted. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution and store at 4°C. Do not freeze.
Storage Buffer
PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide