Application
Cullin 4b antibody detects Cullin 4b protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg Raw 264.7 whole cell lysate/extract E. 30 μg C2Cl2 whole cell lysate/extract 5 % SDS-PAGE Cullin 4b antibody dilution: 1:1000Application
Cullin 4b antibody detects Cullin 4b protein by western blot analysis. A. 30 μg A375 whole cell lysate/extract 5 % SDS-PAGE Cullin 4b antibody dilution: 1:1000Application
Cullin 4b antibody detects Cullin 4b protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cullin 4b protein stained by Cullin 4b antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Cullin 4b antibody detects Cullin 4b protein at nucleus on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma. Cullin 4b antibody dilution: 1:1000.Application
Cullin 4b antibody detects Cullin 4b protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE Cullin 4b antibody dilution: 1:1000Application
Cullin 4b antibody detects Cullin 4b protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE Cullin 4b antibody dilution: 1:1000| Product Name | Cullin 4b antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cullin-4b (cullin 4b)This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cullin 4b. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MRXS15 MRXSC MRXHF2 SFM2 CUL4B cullin 4B |
|---|---|
| Target | Cullin 4B |
| Molecular Weight(MW) | 104 kDa (note) |
Application
Cullin 4b antibody detects Cullin 4b protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg Raw 264.7 whole cell lysate/extract E. 30 μg C2Cl2 whole cell lysate/extract 5 % SDS-PAGE Cullin 4b antibody dilution: 1:1000
Application
Cullin 4b antibody detects Cullin 4b protein by western blot analysis. A. 30 μg A375 whole cell lysate/extract 5 % SDS-PAGE Cullin 4b antibody dilution: 1:1000
Application
Cullin 4b antibody detects Cullin 4b protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cullin 4b protein stained by Cullin 4b antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Cullin 4b antibody detects Cullin 4b protein at nucleus on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma. Cullin 4b antibody dilution: 1:1000.
Application
Cullin 4b antibody detects Cullin 4b protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE Cullin 4b antibody dilution: 1:1000
Application
Cullin 4b antibody detects Cullin 4b protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE Cullin 4b antibody dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
