Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cullin 5 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Cullin 5 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:10000| Product Name | Cullin 5 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cullin 5 (cullin 5)Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. Seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Cullin 5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Cat, RabbitMouse (95%), Rat (95%), Xenopus laevis (99%), Cat (99%), Rabbit (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | VACM-1 antibody VACM1 antibody CUL5 antibody vasopressin-activated calcium-mobilizing receptor 1 antibody CUL-5 antibody cullin-5 antibody Vasopressin-activated calcium-mobilizing receptor-1 antibody Cullin-5 (vasopressin-activated calcium-mobilizin |
|---|---|
| Molecular Weight(MW) | 91 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cullin 5 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Cullin 5 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:10000| Positive Control | NT2D1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
