Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using CUTC antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:2000Application
CUTC antibody [N1C2] detects CUTC protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTC protein stained by CUTC antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | CUTC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CUTC (cutC copper transporter homolog (E. coli))Members of the CUT family of copper transporters are associated with copper homeostasis and are involved in the uptake, storage, delivery, and efflux of copper (Gupta et al., 1995 [PubMed 7635807]; Li et al., 2005 [PubMed 16182249]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CUTC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, BovineMouse (95%), Rat (91%), Xenopus laevis (81%), Chicken (86%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RP11-483F11.3 antibody CUTC antibody copper homeostasis protein cutC homolog antibody cutC copper transporter homolog (E. coli) antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using CUTC antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:2000
Application
CUTC antibody [N1C2] detects CUTC protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTC protein stained by CUTC antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | A549 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
