Application
CUTL1 antibody detects CUTL1 protein at Golgi apparatus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTL1 protein stained by CUTL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
CUTL1 antibody immunoprecipitates CUTL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of CUTL1 protein by 2 μg of CUTL1 antibody 7.5% SDS-PAGE The immunoprecipitated CUTL1 protein was detected by CUTL1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: Raji B: K562 5% SDS PAGE diluted at 1:500| Product Name | CUTL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CUTL1 (cut-like homeobox 1)The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CUTL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CASP antibody CDP antibody CDP/Cut antibody CDP1 antibody COY1 antibody CUTL1 antibody CUX antibody Clox antibody Cux/CDP antibody FLJ31745 antibody GOLIM6 antibody Nbla10317 antibody p100 antibody p110 antibody p200 antibody p75 antibody C |
|---|---|
| Molecular Weight(MW) | 164kDa, 77kDa (note) (6) |
| Cellular Localization | Golgi apparatus membrane |
Application
CUTL1 antibody detects CUTL1 protein at Golgi apparatus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTL1 protein stained by CUTL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
CUTL1 antibody immunoprecipitates CUTL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of CUTL1 protein by 2 μg of CUTL1 antibody 7.5% SDS-PAGE The immunoprecipitated CUTL1 protein was detected by CUTL1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: Raji B: K562 5% SDS PAGE diluted at 1:500| Positive Control | Raji , K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
