Application
Western blot analysis of KC in transfected cells.WB
(B) Western blot analysis of the relative levels of CXCL1 to the control β-actin expression in the lungs of rats.IF
(C) Immunofluorescent analysis of CXCL1 expression in lung resident macrophages using anti-CXCL1 (red) and anti-F4/80 (green) (200 × magnification).| Product Name | CXCL1 / GRO alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CXCL1 / GRO alphaKC, a homolog of human and hamster gro/MGSA, is a 72-amino acid CXC chemokine originally cloned from rat macrophages and lung tissue. It is the mediator for recruitment and activation of netrophils in rat lung inflammation models. Expression of KC can be upregulated by LPS and IL-1beta stimulation. IFN-g blocks LPS-induced expression of KC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCIPNeutWB |
| Western blot analysis (0.5-4 µg/ml), Immunoprecipitation (3-5 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.: |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KC GRO alpha chemokine (C-X-C) GRO chemokine (CXC) chemokine (C X C) |
|---|---|
| Target | KC |
| Tissue Specificity | This antibody is specific for KC. |
Application
Western blot analysis of KC in transfected cells.
WB
(B) Western blot analysis of the relative levels of CXCL1 to the control β-actin expression in the lungs of rats.
IF
(C) Immunofluorescent analysis of CXCL1 expression in lung resident macrophages using anti-CXCL1 (red) and anti-F4/80 (green) (200 × magnification).| Application Notes | Western blot analysis (0.5-4 µg/ml), Immunoprecipitation (3-5 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% thimerosal |
