Application
IHC-P analysis of human colon/rectum adenocarcinoma using CXCL5 antibody. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.| Product Name | CXCL5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CXCL5The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant Human ENA-78 (CXCL5) (5-78 a.a.) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Recommended Starting Dilutions:
For WB: Use at a concenration of 0.1- 0.2 µg/ml.
For IHC-P: Use at a concentration of 1.0 –2.0 μg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml.
Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ENA-78 chemokine (C-X-C motif) ligand 5 SCYB5 |
|---|---|
| Target | CXCL5 |
Application
IHC-P analysis of human colon/rectum adenocarcinoma using CXCL5 antibody. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.| Application Notes | Recommended Starting Dilutions:
For WB: Use at a concenration of 0.1- 0.2 µg/ml.
For IHC-P: Use at a concentration of 1.0 –2.0 μg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml.
Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2 |
