Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed Hep G2, using CXCR1 antibody at 1:500 dilution.Application
CXCR1 antibody [C2C3], C-term detects IL8RA protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. CXCR1 antibody [C2C3], C-term dilution: 1:500.Application
CXCR1 antibody [C2C3], C-term detects CXCR1 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: CXCR1 antibody [C2C3], C-term dilution: 1:250.Application
Untreated (–) and treated (+) mouse tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR1 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (CFA: Complete Freunds adjuvant)| Product Name | CXCR1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CXCR1 (chemokine (C-X-C motif) receptor 1)The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CXCR1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C-C antibody C-C-CKR-1 antibody CD128 antibody CD181 antibody CDw128a antibody CKR-1 antibody CMKAR1 antibody IL8R1 antibody IL8RA antibody IL8RBA antibody CXCR1 antibody interleukin-8 receptor type 1 antibody interleukin 8 receptor alpha anti |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) |
| Cellular Localization | Cell membrane |
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed Hep G2, using CXCR1 antibody at 1:500 dilution.
Application
CXCR1 antibody [C2C3], C-term detects IL8RA protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. CXCR1 antibody [C2C3], C-term dilution: 1:500.
Application
CXCR1 antibody [C2C3], C-term detects CXCR1 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: CXCR1 antibody [C2C3], C-term dilution: 1:250.
Application
Untreated (–) and treated (+) mouse tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR1 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (CFA: Complete Freunds adjuvant)| Positive Control | HepG2 , mouse leukocyte treated with CFA |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
