| Product Name | CXCR3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [49801.111] to CXCR3Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX 3 CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX 3 CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient.CXCR3, also known as the IP10/ Mig receptor, shares approximately 40% protein sequence identity with CXCR1 and CXCR2, and 34. 2 -36.9% amino acid sequence identity with the five known CC chemokine receptors 1-5. CXCR3 is highly expressed by IL2 activated T lymphocytes (Th1), but not by resting T lymphocytes, B lymphocytes, monocytes or granulocytes. CXCR3 binds IP10 and Mig (but not PF4) with high affinity and mediates Ca 2+ mobilization and chemotaxis. CXCR3 does not bind any of the CXC chemokines containing the ELR motif. |
| Immunogen | Human CXCR3 transfected NSO mouse myeloma cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 49801.111 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-P |
| FACS: Use 5μg for 10^5 cells. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 600894 IP10-R IP10 Mig-R CXCR3 CKR-L2 2833 IP10R P49682 CKRL2 CD183 CMKAR3 GPR9 MigR Mig R IP10 R CKR L2 |
|---|---|
| Target | CXCR3 |
| Tissue Specificity | This product reacts with CXCR3 transfected cells and not with the parent cell line by flow cytometry. The antibody shows no cross-reactivity with human CXCR1, CXCR2 and CXCR4. |
| Cellular Localization | Integral membrane protein |
| Application Notes | FACS: Use 5μg for 10^5 cells. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None Constituents: PBS Endotoxin level is |
