Application
CXCR3 antibody [N1], N-term detects CXCR3 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CXCR3 antibody [N1], N-term diluted at 1:500.Application
CXCR3 antibody detects CXCR3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR3 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CXCR3 antibody [N1], N-term detects CXCR3 protein at cytoplasm and membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CXCR3 antibody [N1], N-term diluted at 1:500.| Product Name | CXCR3 antibody , N-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CXCR3 (chemokine (C-X-C motif) receptor 3)This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CXCR3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD182 antibody CD183 antibody CKR-L2 antibody CMKAR3 antibody GPR9 antibody IP10-R antibody Mig-R antibody MigR antibody CXCR3 antibody CXC-R3 antibody chemokine (C-X-C) receptor 3 antibody C-X-C chemokine receptor type 3 antibody IP10 receptor |
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| Molecular Weight(MW) | 41 kDa (note) |
| Cellular Localization | Cell membrane |
Application
CXCR3 antibody [N1], N-term detects CXCR3 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CXCR3 antibody [N1], N-term diluted at 1:500.
Application
CXCR3 antibody detects CXCR3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR3 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CXCR3 antibody [N1], N-term detects CXCR3 protein at cytoplasm and membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CXCR3 antibody [N1], N-term diluted at 1:500.| Positive Control | K562 , NCI-H929 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
