Application
Western Blot expression of Cyclin A in HeLA (B) whole cell lysates| Product Name | Cyclin A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cyclin ADuring each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9). |
| Immunogen | Synthetic peptide: HSKYHSVSLLNPPETLSV (Mouse) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-FIPWB |
| IHC:1:10~1:50 IHC-F:1:10~1:20 IP:1:20~1:100 WB:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Ccna CCNA2 Cyca P20248 12428 AA408589 890 CCN1 Cyclin A Ccn-1 123835 Ccn 1 |
|---|---|
| Target | Cyclin A |
| Tissue Specificity | Reacts with cyclin A2 |
| Cellular Localization | Nuclear |
Application
Western Blot expression of Cyclin A in HeLA (B) whole cell lysates| Positive Control | K562 |
|---|---|
| Application Notes | IHC:1:10~1:50 IHC-F:1:10~1:20 IP:1:20~1:100 WB:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store this product at 4 °C, do not freeze. The product is stable for one year from the date of shipment. |
| Storage Buffer | PBS with 0.1% sodium azide |
