| Product Name | Cyclin A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cyclin ACyclins are regulatory subunits of the cyclin dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2 phase. |
| Immunogen | Synthetic peptide: C-KYHGVSLLNPPETLNL, corresponding to amino acids 417-432 of Human Cyclin A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Immunoprecipitation (with protein A, native and denatured): use at a concentration of 10μg / mg of lysate (co-precipitates cdk 2 / Cyclin A complex). Western Blotting: use at a concentration of 5μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 60 kDa, which corresponds to the predicted molecular weight of Cyclin A.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | CCNA2 P20248 CCNA 890 CCN1 Cyclin A 123835 |
|---|---|
| Target | Cyclin A |
| Cellular Localization | Nuclear |
| Positive Control | HeLa cells |
|---|---|
| Application Notes | Immunoprecipitation (with protein A, native and denatured): use at a concentration of 10μg / mg of lysate (co-precipitates cdk 2 / Cyclin A complex). Western Blotting: use at a concentration of 5μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 60 kDa, which corresponds to the predicted molecular weight of Cyclin A.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
