Application
Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. Cyclin B1 antibody diluted at 1:500.Application
Sample (50 µg of HeLa whole cell lysate) diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Western blot analysis of cyclin B1 ( upper panel) and beta-actin ( lower panel) Sample (30 µg of whole cell lysate) A: U2OS B: U2OS treated 100ng/ml Nocodazole 16 hr 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Cyclin B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin B1 (cyclin B1)The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (85%), Rhesus Monkey (97%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCNB antibody CCNB1 antibody G2/mitotic-specific cyclin-B1 antibody G2/mitotic-specific cyclin B1 antibody cyclin B1 antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , Centrosome |
Application
Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. Cyclin B1 antibody diluted at 1:500.
Application
Sample (50 µg of HeLa whole cell lysate) diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Western blot analysis of cyclin B1 ( upper panel) and beta-actin ( lower panel) Sample (30 µg of whole cell lysate) A: U2OS B: U2OS treated 100ng/ml Nocodazole 16 hr 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | U2OS , U2OS treated 100ng/ml Nocodazole 16hr , Hela , *T84 , *CL1-5 , *CL1-0 , *A549 , *HUVEC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
