Application
Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).Application
Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.| Product Name | Cyclin B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5G6] to CCNB1The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human CCNB1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5G6 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Gene Synonyms | CCNB1 CCNB |
|---|---|
| Target | CCNB1 |
| Molecular Weight(MW) | 60 kDa (note) |
Application
Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).
Application
Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
