Application
Western blot analysis of Cyclin D1 in Cos17 cell extract usingApplication
Western blot analysis of Cyclin B1 in Cos17 cell extract using| Product Name | Cyclin B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [GNS1] to Cyclin B1In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at aa 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34 cdc2 rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. |
| Immunogen | Recombinant protein corresponding to human Cyclin B1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GNS1 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a concentration of 1-2µg/ml for 2hrs at RT. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCNB1 Cyclin B1 891 123836 P14635 CCNB |
|---|---|
| Target | Cyclin B1 |
Application
Western blot analysis of Cyclin D1 in Cos17 cell extract using
Application
Western blot analysis of Cyclin B1 in Cos17 cell extract using| Positive Control | MAD109 or LS174T cells |
|---|---|
| Application Notes | For WB: Use at a concentration of 1-2µg/ml for 2hrs at RT. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH7.4 containing 0.2% BSA and 0.09% sodium azide |
