Application
Application| Product Name | Cyclin B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [V152] to Cyclin B1In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition. |
| Immunogen | Tagged hamster Cyclin B1 expressed in bacteria, harvested from inclusion bodies, extracted with 6M guanidine HCl and purified by affinity chromatograaphy. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | V152 |
| Host Species | Mouse |
| Tested Applications | AFFACSICC/IFIHCIHC-FIHC-PIPWB |
| Very good at detecting cyclin B1 in formalin-fixed tissues. Flow cytometry / FACS: assay dependent. Western Blotting: use at a concentration of 1 - 2μg/ml for 2hrs at RT. Immunohistochemistry: use at a concentration of 1 - 2μg/ml for 30 min at RT. Immunofluorescence : 2 micrograms/ml in PBS/ 1% BSA and incubation was overnignt at 4°C. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 62 kDa, which corresponds to the predicted molecular weight of Cyclin B1. Optimal dilutions/concentrations should be determined by the end user: |
|
| Species Reactivity | HumanMouseHamster |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC90915 CCNB1 Cyclin B1 891 MGC18763 Cycb-4 P14635 Cycb4 CCNB Ccnb1-rs13 Ccnb1rs13 268697 123836 Cycb 4 Ccnb1 rs13 |
|---|---|
| Target | Cyclin B1 |
| Tissue Specificity | This antibody is specific to a protein of 62 kD identified as cyclin B1. Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression. |
| Cellular Localization | Nuclear |
Application
Application| Positive Control | Jurkat cells Tonsil or breast carcinoma |
|---|---|
| Application Notes | Very good at detecting cyclin B1 in formalin-fixed tissues. Flow cytometry / FACS: assay dependent. Western Blotting: use at a concentration of 1 - 2μg/ml for 2hrs at RT. Immunohistochemistry: use at a concentration of 1 - 2μg/ml for 30 min at RT. Immunofluorescence : 2 micrograms/ml in PBS/ 1% BSA and incubation was overnignt at 4°C. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 62 kDa, which corresponds to the predicted molecular weight of Cyclin B1. Optimal dilutions/concentrations should be determined by the end user: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline |
