| Product Name | Cyclin D1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CD1.1] to Cyclin D1Cyclin D1 is a 36kD nuclear protein. D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy, and over expression has been linked to poor prognosis. |
| Immunogen | Purified cyclin D1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CD1.1 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-FIHC-PIPWB |
| Suggested working dilution(s): WB,FACS,ICC/IF,IP,WB: 1μg/ml IHC-Fr: 2μg/ml.IHC-P:2-5ug/ml.Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. For frozen sections - positive tissue: colon.FACS: membrane permeabilization is required. ELISA: dilutions determined by end user. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P24385 PRAD1 CCND1 BCL1 D11S287E Cyclin D1 U21B31 595 151400 |
|---|---|
| Target | Cyclin D1 |
| Tissue Specificity | The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions. |
| Positive Control | Small intestine, colon |
|---|---|
| Application Notes | Suggested working dilution(s): WB,FACS,ICC/IF,IP,WB: 1μg/ml IHC-Fr: 2μg/ml.IHC-P:2-5ug/ml.Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. For frozen sections - positive tissue: colon.FACS: membrane permeabilization is required. ELISA: dilutions determined by end user. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 15 mM sodium azide, approx. pH 7.4 |
