Application
Human uterine mesodermal tumor cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Cyclin D1 (DCS-6) . The antibody was developed with Anti-Mouse IgG (Fab specific), Alkaline Phosphatase conjugate and a NBT/BCIP substrate. Lanes 1. Antibody diluted 1/200 2. Antibody diluted 1/400| Product Name | Cyclin D1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DCS - 6] to Cyclin D1During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks. Early in the G1 phase of the cell cycle cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | DCS-6 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| ICC: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/200. Detects a band of approximately 36 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P24385 PRAD1 CCND1 BCL1 D11S287E Cyclin D1 U21B31 595 151400 |
|---|---|
| Target | Cyclin D1 |
| Tissue Specificity | Does not cross-react with other D-type cyclins. |
| Cellular Localization | Nuclear |
Application
Human uterine mesodermal tumor cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Cyclin D1 (DCS-6) . The antibody was developed with Anti-Mouse IgG (Fab specific), Alkaline Phosphatase conjugate and a NBT/BCIP substrate. Lanes 1. Antibody diluted 1/200 2. Antibody diluted 1/400| Application Notes | ICC: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/200. Detects a band of approximately 36 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at −20°C. |
| Storage Buffer | Preservative: 15mM Sodium Azide |
