Application
ApplicationApplication
IHC Image using - Detection of Cyclin D1 by IHC in Human Breast Carcinoma| Product Name | Cyclin D1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Cyclin D1This antibody is specific to human cyclin D1. Cyclin D1 gene is located on 11q13 and has been shown to be amplified in a variety of tumors. Maximum expression of cyclin D1 occurs at a critical point in mid to late G1 phase of the cell cycle. This antibody cross reacts with monkey, rat and mouse. |
| Immunogen | Prokaryotic recombinant fusion protein corresponding to human cyclin D1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | DCS-6 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P24385 PRAD1 CCND1 BCL1 D11S287E Cyclin D1 U21B31 595 151400 |
|---|---|
| Target | Cyclin D1 |
| Cellular Localization | Nuclear |
Application
Application
Application
IHC Image using - Detection of Cyclin D1 by IHC in Human Breast Carcinoma| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
