Application
Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin D1 antibody (cat. # ).| Product Name | Cyclin D1 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Cyclin D1The cyclin D1 protein belongs to a family of proteins which function primarily in the cell division cycle by regulating the activity of cyclin dependent kinases (cdks). The chromosomal region in which the cyclin D1 gene is located is amplified in a number of cancers. The human cyclin D1 gene has been mapped to the 11q13 locus and has been shown to be the PRAD1 gene product. |
| Immunogen | BALB/C mice were immunized with human recombinant cyclin D1 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DCS-6 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P24385 PRAD1 CCND1 BCL1 D11S287E Cyclin D1 U21B31 595 151400 |
|---|---|
| Target | Cyclin D1 |
| Cellular Localization | Nuclear |
Application
Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin D1 antibody (cat. # ).| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
