Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D2 antibody diluted at 1:1000.Application
Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D2 protein stained by Cyclin D2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Cyclin D2 antibody diluted at 1:500.| Product Name | Cyclin D2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin D2The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cyclin D2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCND2 antibody KIAK0002 antibody |
|---|---|
| Target | Cyclin D2 |
| Molecular Weight(MW) | 33 kDa (note) |
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D2 antibody diluted at 1:1000.
Application
Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D2 protein stained by Cyclin D2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Cyclin D2 antibody diluted at 1:500.| Positive Control | RD |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
