| Product Name | Cyclin D2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DCS - 3.1] to Cyclin D2Cyclin D2 is a G1 cyclin required for G1-phase progression, and is a strong candidate for a proto-oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk4 and/or cdk6. |
| Immunogen | Purified human recombinant full length cyclin D2 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | DCS-3.1 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIPWB |
| For FACS: Use at an assay dependent dilution. For functional studies: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For IP(with protein A): Use at a concentration of 2μg/mg protein lysate (native and denatured). For WB: Use at an assay dependent dilution. By Western blot, this antibody detects a band of ~ 34 kDa, which corresponds to the predicted molecular weight of Cyclin D2. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 123833 KIAK0002 CCND2 Cyclin D2 P30279 894 MGC102758 |
|---|---|
| Target | Cyclin D2 |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
| Positive Control | U-2-OS, C82, RD, Bristol-8, or LoVo36 cells. Please note that T47D, ZR75, SKBR3, MCF-7, BT-20, MDA468, HBL100, BT5549, HeLa, Saos2, and HT29 cells are negative for cyclin D2. |
|---|---|
| Application Notes | For FACS: Use at an assay dependent dilution. For functional studies: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For IP(with protein A): Use at a concentration of 2μg/mg protein lysate (native and denatured). For WB: Use at an assay dependent dilution. By Western blot, this antibody detects a band of ~ 34 kDa, which corresponds to the predicted molecular weight of Cyclin D2. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
