Application
Cyclin D3 antibody detects Cyclin D3 protein at nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. Cyclin D3 antibody diluted at 1:500.Application
Cyclin D3 antibody detects Cyclin D3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D3 protein stained by Cyclin D3 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Application
Cyclin D3 antibody detects Cyclin D3 protein at nucleus in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Cyclin D3 antibody diluted at 1:500.| Product Name | Cyclin D3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin D3 (G1/S-specific cyclin-D3)The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cyclin D3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Sheep, Rhesus Monkey, BovinePig (97%), Sheep (99%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCND3 antibody G1/S-specific cyclin-D3 antibody G1/S-specific cyclin D3 antibody D3-type cyclin antibody cyclin D3 antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Cyclin D3 antibody detects Cyclin D3 protein at nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. Cyclin D3 antibody diluted at 1:500.
Application
Cyclin D3 antibody detects Cyclin D3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D3 protein stained by Cyclin D3 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Application
Cyclin D3 antibody detects Cyclin D3 protein at nucleus in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Cyclin D3 antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
