Application
Detection of Cyclin E by IHC in Human Breast CarcinomaApplication
Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin E, using ABC and DAB chromagen.| Product Name | Cyclin E antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HE12] to Cyclin ECyclin proteins are a family of related proteins that control the progression of the eukaryotic cell cycle. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16. |
| Immunogen | Synthetic peptide corresponding to human cyclin E protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HE12 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| For IHC-P: Use at a dilution of 1:25-1:50 in an ABC method for 30 min at RT. High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 is required prior to immunostaining. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CCNE1 CCNE P24864 898 123837 Cyclin E |
|---|---|
| Target | Cyclin E |
| Cellular Localization | Nuclear |
Application
Detection of Cyclin E by IHC in Human Breast Carcinoma
Application
Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin E, using ABC and DAB chromagen.| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:25-1:50 in an ABC method for 30 min at RT. High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 is required prior to immunostaining. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline containing sodium azide |
