Application
ICC/IF analysis of HeLa cells using Cyclin H antibody (green) and DAPI (blue).Application
WB analysis of extracts from various cell lines using Cyclin H antibody.| Product Name | Cyclin H antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cyclin HThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010] |
| Immunogen | Recombinant protein of human CCNH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:20 - 1:100.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAK CCNH cyclin H p34 p37 |
|---|---|
| Target | Cyclin H |
| Molecular Weight(MW) | 38 kDa (note) |
Application
ICC/IF analysis of HeLa cells using Cyclin H antibody (green) and DAPI (blue).
Application
WB analysis of extracts from various cell lines using Cyclin H antibody.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:20 - 1:100.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
