Application
Immunohistochemical analysis of paraffin-embedded H1299 Xenograft, using cyclin T2 antibody at 1:100 dilution.Application
Cyclin T2 antibody [C2C3], C-term detects cyclin T2 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Cyclin T2 antibody [C2C3], C-term diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using cyclin T2 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000| Product Name | Cyclin T2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to cyclin T2 (cyclin T2)The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Two alternatively spliced transcript variants, which encode distinct isoforms, have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human cyclin T2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (80%), Bovine (95%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYCT2 antibody FLJ13583 antibody FLJ90560 antibody MGC134840 antibody CCNT2 antibody SDS-stable vimentin-bound DNA fragment HEF42VIM22 antibody cyclin-T2 antibody subunit of positive elongation transcription factor b antibody cyclin T2b antibody c |
|---|---|
| Target | Cyclin T2 |
| Molecular Weight(MW) | 81 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded H1299 Xenograft, using cyclin T2 antibody at 1:100 dilution.
Application
Cyclin T2 antibody [C2C3], C-term detects cyclin T2 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Cyclin T2 antibody [C2C3], C-term diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using cyclin T2 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
