Application
Western blot of CyP-40 on rat brain extract using| Product Name | Cyclophilin 40 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclophilin 40Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues. |
| Immunogen | Full length, recombinant human Cyclophilin 40 expressed in E. coli. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | GSAWB |
| WB: 1:1,000 Optimal dilution / concentration should be determined by the end user.: |
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| Species Reactivity | HumanMouseRatAmphibians |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 601753 Cyclophilin40 MGC33096 CYP-40 PPID CYPD 5481 CYP40 Cyclophilin 40 Q08752 CYP 40 |
|---|---|
| Target | Cyclophilin 40 |
Application
Western blot of CyP-40 on rat brain extract using| Application Notes | WB: 1:1,000 Optimal dilution / concentration should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% sodium azide. |
