Application
By Western blot, this antibody detects a 40 kDa protein representing CyP-40 from rat brain extract.Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human Hepatocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing Cyclophilin D or without primary antibody (negative control) overnight at 4°C in a humidified chamber.Application
Immunohistochemistry was performed on normal deparaffinized human Skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Cyclophilin D or without primary antibody (negative control) overnight at 4°C in a humidified chamber.| Product Name | Cyclophilin 40 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclophilin 40Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues. |
| Immunogen | Synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyclophilin 40. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | FACSGSAICC/IFIHCIHC-FIHC-PIPWB |
| ICC/IF: 1:200 - 1:250: |
|
| Species Reactivity | HumanMouseChickenRatPrimateRabbitBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Ppid 4930564J03Rik CYP-40 Ppidl Ppif peptidylprolyl isomerase D (cyclophilin D) |
|---|---|
| Target | Cyclophilin 40 |
Application
By Western blot, this antibody detects a 40 kDa protein representing CyP-40 from rat brain extract.
Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human Hepatocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing Cyclophilin D or without primary antibody (negative control) overnight at 4°C in a humidified chamber.
Application
Immunohistochemistry was performed on normal deparaffinized human Skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Cyclophilin D or without primary antibody (negative control) overnight at 4°C in a humidified chamber.| Application Notes | ICC/IF: 1:200 - 1:250: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% sodium azide. |
