Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cyclophilin A antibody at 1:100 dilution.Application
Cyclophilin A antibody detects Cyclophilin A protein by western blot analysis. Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Cyclophilin A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclophilin A (peptidylprolyl isomerase A (cyclophilin A))This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cyclophilin A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Cat, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, BovineRat (96%), Cat (97%), Pig (99%), Rabbit (96%), Chicken (92%), Sheep (99%), Rhesus Monkey (100%), Bovine (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CYPA antibody CYPH antibody MGC117158 antibody MGC12404 antibody MGC23397 antibody PPIA antibody cyclophilin antibody PPIase A antibody rotamase A antibody peptidyl-prolyl cis-trans isomerase A antibody cyclosporin A-binding protein antibody T c |
|---|---|
| Molecular Weight(MW) | 18 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cyclophilin A antibody at 1:100 dilution.
Application
Cyclophilin A antibody detects Cyclophilin A protein by western blot analysis. Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Raji WCE , Raji C+M , Raji NE , Molt-4 , Raji , Mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
