Application
Immunofluorescence analysis of methanol-fixed HeLa, using c-Yes antibody at 1:200 dilution.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000| Product Name | c-Yes antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to c-Yes (v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1)This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human c-Yes. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Chicken, Rhesus Monkey, BovineZebrafish (95%), Xenopus laevis (97%), Dog (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HsT441 antibody P61-YES antibody Yes antibody c-yes antibody YES1 antibody proto-oncogene c-Yes antibody tyrosine-protein kinase Yes antibody Yamaguchi sarcoma oncogene antibody cellular yes-1 protein antibody proto-oncogene tyrosine-protein kinas |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using c-Yes antibody at 1:200 dilution.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
