Application
Immunoprecipitation of CYFIP1 protein from HeLa whole cell extracts using 5 μg of CYFIP1 antibody [N1N2], N-term . Western blot analysis was performed using CYFIP1 antibody [N1N2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 µg of whole cell lysate) A: BCL-1 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using CYFIP1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CYFIP1 antibody at 1:500 dilution.| Product Name | CYFIP1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYFIP1 (cytoplasmic FMR1-interacting protein 1)Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CYFIP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, BovineRat (100%), Zebrafish (93%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ45151 antibody P140SRA-1 antibody SHYC antibody SRA1 antibody CYFIP1 antibody sra-1 antibody cytoplasmic FMRP interacting protein 1 antibody selective hybridizing clone antibody specifically Rac1-associated protein 1 antibody cytoplasmic FMR1-i |
|---|---|
| Molecular Weight(MW) | 145 kDa (note) (7) |
| Cellular Localization | Cytoplasm , perinuclear region , Cell projection , lamellipodium , ruffle , Cell junction , synapse , synaptosome |
Application
Immunoprecipitation of CYFIP1 protein from HeLa whole cell extracts using 5 μg of CYFIP1 antibody [N1N2], N-term . Western blot analysis was performed using CYFIP1 antibody [N1N2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 µg of whole cell lysate) A: BCL-1 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using CYFIP1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CYFIP1 antibody at 1:500 dilution.| Positive Control | HeLa , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
