Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using cylindromatosis 1 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using cylindromatosis 1 antibody at 1:200 dilution.Application
CYLD antibody detects CYLD protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE CYLD antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CYLD antibody detects CYLD protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE CYLD antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CYLD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYLD (cylindromatosis (turban tumor syndrome))This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CYLD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDMT antibody CYLD1 antibody CYLDI antibody EAC antibody FLJ20180 antibody FLJ31664 antibody FLJ78684 antibody KIAA0849 antibody MFT antibody MFT1 antibody SBS antibody TEM antibody USPL2 antibody CYLD antibody deubiquitinating enzyme CYLD an |
|---|---|
| Target | cylindromatosis 1 |
| Molecular Weight(MW) | 107 kDa (note) (3) |
| Cellular Localization | Cytoplasm , perinuclear region |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using cylindromatosis 1 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using cylindromatosis 1 antibody at 1:200 dilution.
Application
CYLD antibody detects CYLD protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE CYLD antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CYLD antibody detects CYLD protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE CYLD antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
