Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CYP1A2 mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2.Application
Immunofluorescent staining of HT29 cells using anti-CYP1A2 mouse monoclonal antibody .Application
Anti-CYP1A2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CYP1A2Application
HEK293T cells transfected with either RC221636 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP1A2 antibody, and then analyzed by flow cytometry.Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CYP1A2 mouse monoclonal antibody. ( Dilution 1:50)| Product Name | CYP1A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7D12] to CYP1A2Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. |
| Immunogen | Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 7D12 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYP1A2 CP12 P450(PA) P3450 P3-450 |
|---|---|
| Target | CYP1A2 |
| Tissue Specificity | is specific for human CYP1A2 (NP_000752). |
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CYP1A2 mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2.
Application
Immunofluorescent staining of HT29 cells using anti-CYP1A2 mouse monoclonal antibody .
Application
Anti-CYP1A2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CYP1A2
Application
HEK293T cells transfected with either RC221636 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYP1A2 antibody, and then analyzed by flow cytometry.
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CYP1A2 mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
