Application
Confocal immunofluorescent analysis of CYP2C19 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP2C19 Antibody (N-term),which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of CYP2C19 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). CYP2C19 (arrow) was detected using the purified Pab.| Product Name | CYP2C19 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CYP2C19 (N-term)This gene encodes a member of the cytochrome P45 superfamily of enzymes. The cytochrome P45 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P45 genes on chromosome 1q24. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 88~117 amino acids from the N-terminal region of human CYP2C19. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | P45011A (S)-limonene 7-monooxygenase Cytochrome P450 2C19 (S)limonene7monooxygenase CYPIIC19 P450-254C (S)limonene 6monooxygenase CYPIIC17 Mephenytoin 4hydroxylase Mephenytoin 4-hydroxylase CYP2C19 P450254C Cytochrome P4502C19 Mephenytoin4hydr |
|---|---|
| Target | CYP2C19 |
| Molecular Weight(MW) | 55.931 kDa (note) |
Application
Confocal immunofluorescent analysis of CYP2C19 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP2C19 Antibody (N-term),which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of CYP2C19 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). CYP2C19 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
