Application
Paraffin embedded sections of human breast cancer tissue were incubated with anti-human CYR61 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)Application
The cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CYR61 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | CYR61 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3H3 ] to CYR61Cysteine-rich angiogenic inducer 61, also known as CYR61, is a growth factor-inducible, immediate-early gene that has multifaceted activities in various cancers. CYR61 is a secreted heparin-binding extracellular matrix-associated protein whose activities include the promotion of adhesion and chemotaxis, and the stimulation of fibroblast and endothelial cell growth. CYR61 induces angiogenesis and promotes tumor growth and is expressed in dermal fibroblasts during cutaneous wound healing. |
| Immunogen | Recombinant human CYR61 (168-242aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H3 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| Western Blot: Use at 1:250~1:1,000 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCN family member 1 IBP10 cysteinerich angiogenic inducer 61 CCN1 CCN family member1 CYR61 cysteinerich angiogenic inducer61 IBP-10 GIG1 cysteine-rich angiogenic inducer 61 IBP 10 |
|---|---|
| Target | CYR61 |
| Light Chain Type | kappa |
Application
Paraffin embedded sections of human breast cancer tissue were incubated with anti-human CYR61 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Application
The cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CYR61 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:250~1:1,000 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
