Application
Cystatin B antibody detects Cystatin B protein at cytoplasm and nucleus human on tonsil by immunohistochemical analysis. Sample: Paraffin-embedded tonsil. Cystatin B antibody dilution: 1:100.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Cystatin B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Cystatin B antibody detects Cystatin B protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Cystatin B antibody diluted at 1:100.| Product Name | Cystatin B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cystatin B (cystatin B (stefin B))The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cystatin B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Rhesus Monkey, ChimpanzeePig (81%), Rhesus Monkey (95%), Chimpanzee (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CST6 antibody EPM1 antibody PME antibody STFB antibody CSTB antibody stefin-B antibody CPI-B antibody cystatin-B antibody stefin B antibody liver thiol proteinase inhibitor antibody cystatin B (stefin B) antibody |
|---|---|
| Molecular Weight(MW) | 11 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Cystatin B antibody detects Cystatin B protein at cytoplasm and nucleus human on tonsil by immunohistochemical analysis. Sample: Paraffin-embedded tonsil. Cystatin B antibody dilution: 1:100.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Cystatin B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Cystatin B antibody detects Cystatin B protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Cystatin B antibody diluted at 1:100.| Positive Control | Target recombinant protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
