Application
Human tissue extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin C antibody diluted at 1:1000.Application
Cystatin C antibody detects Cystatin C protein at cytoplasm in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. Cystatin C antibody diluted at 1:400.Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin C antibody diluted at 1:1000.| Product Name | Cystatin C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cystatin C (cystatin C)The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cystatin C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (96%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARMD11 antibody CST3 antibody Cystatin C antibody Cystatin C antibody |
|---|---|
| Target | Cystatin C |
| Molecular Weight(MW) | 16 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Human tissue extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin C antibody diluted at 1:1000.
Application
Cystatin C antibody detects Cystatin C protein at cytoplasm in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. Cystatin C antibody diluted at 1:400.
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin C antibody diluted at 1:1000.| Positive Control | A431 , HepG2 , Human plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
