Application
Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cystatin S antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed A431, using Cystatin S antibody at 1:200 dilution.| Product Name | Cystatin S antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cystatin S (cystatin S)The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cystatin S. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC71923 antibody CST4 antibody cystatin-SA-III antibody cystatin-4 antibody salivary acidic protein 1 antibody cystatin-S antibody cystatin 4 antibody cystatin S antibody |
|---|---|
| Molecular Weight(MW) | 16 kDa (note) |
| Cellular Localization | Secreted |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cystatin S antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed A431, using Cystatin S antibody at 1:200 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
