Application
Western Blot analysis of CYB5A expression in transfected 293T cell line by CYB5A polyclonal antibody. A: Non-transfected lysate. B: CYB5A transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | Cytochrome b5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cytochrome b5 (cytochrome b5 type A (microsomal))The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytochrome b5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Chicken, Sheep, Rhesus Monkey, BovineMouse (88%), Rat (88%), Xenopus laevis (80%), Dog (95%), Pig (92%), Chicken (82%), Sheep (92%), Rhesus Monkey (97%), Bovine (91%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYB5 antibody MCB5 antibody CYB5A antibody type 1 cyt-b5 antibody cytochrome b5 antibody cytochrome b5 type A (microsomal) antibody |
|---|---|
| Target | Cytochrome b5 |
| Molecular Weight(MW) | 15 kDa (note) (2) |
| Cellular Localization | Isoform 1: Endoplasmic reticulum membrane , Microsome membrane , Isoform 2: Cytoplasm |
Application
Western Blot analysis of CYB5A expression in transfected 293T cell line by CYB5A polyclonal antibody. A: Non-transfected lysate. B: CYB5A transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | Cytochrome b5 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
