Application
Sample (30 µg of whole cell lysate) A: PC-12 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: K562 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cytochrome C antibody detects Cytochrome C protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Cytochrome C antibody diluted at 1:200.| Product Name | Cytochrome C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytochrome C (cytochrome c, somatic)This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq] |
| Immunogen | Full length human Cytochrome C Recombinant protein. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| WB:1:500~1:2000 IHC:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Chicken, Chimpanzee, BovineXenopus laevis (84%), Dog (89%), Pig (90%), Chicken (87%), Chimpanzee (100%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CYC antibody HCS antibody THC4 antibody CYCS antibody cytochrome c antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
Sample (30 µg of whole cell lysate) A: PC-12 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: K562 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cytochrome C antibody detects Cytochrome C protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Cytochrome C antibody diluted at 1:200.| Positive Control | K562 , Mouse brain , PC-12 , *293T , *SCC-25 |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
