Application
Western blot analysis of Cytochrome P450 2D6 expression in A431 (A); K562 (B); HuvEc (C) whole cell lysates.| Product Name | Cytochrome P450 2D6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytochrome P450 2D6This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 25% of commonly prescribed drugs. Its substrates include antidepressants, antipsychotics, analgesics and antitussives, beta adrenergic blocking agents, antiarrythmics and antiemetics. The gene is highly polymorphic in the human population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Some individuals with the poor metabolizer phenotype have no functional protein since they carry 2 null alleles whereas in other individuals the gene is absent. This gene can vary in copy number and individuals with the ultrarapid metabolizer phenotype can have 3 or more active copies of the gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 2D6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P450C2D CYP2D8P2 CYP2D CYPIID6 CYP2D7P2 P450DB1 CYP2DL1 CYP2D7AP cytochrome P450 family 2 subfamily D member 6 CPD6 CYP2D6 CYP2D7BP |
|---|---|
| Target | Cytochrome P450 2D6 |
| Tissue Specificity | Recognizes endogenous levels of Cytochrome P450 2D6 protein. |
Application
Western blot analysis of Cytochrome P450 2D6 expression in A431 (A); K562 (B); HuvEc (C) whole cell lysates.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
