ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using CYP2D6 antibody against C2C12(1),P19(2),Pc-12(3),Rat heart tissue(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-CYP2D6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CYP2D6 |
|---|---|
| Gene Synonyms | CPD6 CYP2D Cyp2d13 Cyp2d18 Cyp2d22 Cyp2d4v1 Cyp2d4v2 Cyp2d6 CYP2D7AP CYP2D7BP CYP2D7P2 CYP2D8P2 CYP2DL1 CYPIID6 P450-DB1 P450C2D P450DB1 |
| Gene Full Name | cytochrome P450 family 2 subfamily D member 6 (gene/pseudogene) |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 25% of commonly prescribed drugs. Its substrates include antidepressants, antipsychotics, analgesics and antitussives, beta adrenergic blocking agents, antiarrythmics and antiemetics. The gene is highly polymorphic in the human population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Some individuals with the poor metabolizer phenotype have no functional protein since they carry 2 null alleles whereas in other individuals the gene is absent. This gene can vary in copy number and individuals with the ultrarapid metabolizer phenotype can have 3 or more active copies of the gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| Alternative Names | cholesterol 25-hydroxylase CYP2D6 CYPIID18 CYPIID3 CYPIID4 cytochrome P-450 IID3 cytochrome P450 cytochrome P450 2D-29 cytochrome P450 2D-35 cytochrome P450 2d18 cytochrome P450 family 2 subfamily d polypeptide 13 cytochrome P450 family 2 subfamily d polypeptide 22 cytochrome P450 family 2 subfamily D polypeptide 6 cytochrome P450 subfamily 2D polypeptide 6 cytochrome P450 subfamily IID (debrisoquine sparteine etc. -metabolizing) polypeptide 6 cytochrome P450 subfamily IID (debrisoquine sparteine etc. -metabolizing)-like 1 cytochrome P450 subfamily IID polypeptide 6 Cytochrome P450 subfamily IID3 Cytochrome P450 subfamily IID4 cytochrome P450-CMF3 cytochrome P450-DB1 cytochrome P450-DB3 cytochrome P450-DB4 debrisoquine 4-hydroxylase flavoprotein-linked monooxygenase microsomal monooxygenase nonfunctional cytochrome P450 2D6 P450 2D-29/2D-35 P450-CMF3 P450-DB3 P450-DB4 xenobiotic monooxygenase |
| Molecular Weight(MW) | 55kDa |
| Source | Rabbit |
| Cellular Localization | Endoplasmic reticulum membrane;Microsome membrane |
| SwissProt ID | P10635 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using CYP2D6 antibody against C2C12(1),P19(2),Pc-12(3),Rat heart tissue(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
