Application
Immunofluorescence analysis of methanol-fixed A431, using Cytoglobin antibody at 1:500 dilution.Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cytoglobin antibody diluted at 1:1000.Application
Sample (50 ug of whole cell lysate) A: mouse heart 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:2000| Product Name | Cytoglobin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytoglobin (cytoglobin)Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002 [PubMed 11893755]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytoglobin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Sheep, Rhesus Monkey, BovineDog (82%), Sheep (96%), Rhesus Monkey (97%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HGB antibody STAP antibody CYGB antibody histoglobin antibody stellate cell activation-associated protein antibody cytoglobin antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed A431, using Cytoglobin antibody at 1:500 dilution.
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cytoglobin antibody diluted at 1:1000.
Application
Sample (50 ug of whole cell lysate) A: mouse heart 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:2000| Positive Control | IMR32 , mouse heart , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
