Application
Immunohistochemistry staining of skin basaliom (paraffin-embedded sections) with anti-human Cytokeratin 10 .| Product Name | Cytokeratin 10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [VIK - 10] to Cytokeratin 10Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. |
| Immunogen | Tissue / cell preparation (Human). (Cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | VIK-10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| ICC: Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IHC-Fr: Use at an assay dependant concentration. IP: Use at an assay dependant dilution. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Cytokeratin10 K10 P13645 3858 KRT10 Cytokeratin 10 113800 KPP CK10 |
|---|---|
| Target | Cytokeratin 10 |
| Tissue Specificity | This antibody is strongly positive in all suprabasal layers of human epidermis. |
| Cellular Localization | Cytoplasmic |
Application
Immunohistochemistry staining of skin basaliom (paraffin-embedded sections) with anti-human Cytokeratin 10 .| Application Notes | ICC: Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IHC-Fr: Use at an assay dependant concentration. IP: Use at an assay dependant dilution. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
