| Product Name | Cytokeratin 14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [SPK-14.2] to Cytokeratin 14Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. |
| Immunogen | Human cellus keratin amino acids 459-472 of Cytokeratin 14 of humanorigin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | SPK-14.2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| For IF: Use at a dilution of 1:50-1:100. For WB: Use at a dilution of 1:100-1:200. For FACS: Use at an assay dependent dilution. This antibody is used in research to distinguish stratified epithelial cells from simple epithelial cells and identification of squamous cell carcinomas. The optimal conditions should be determined by the individual laboratory.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 3861 Cytokeratin14 CK14 P02533 EBS3 Cytokeratin 14 KRT14 EBS4 K14 131800 |
|---|---|
| Target | Cytokeratin 14 |
| Tissue Specificity | This antibody reacts with the 50 kD cytokeratin 14 polypeptide. |
| Positive Control | Normal Human Skin |
|---|---|
| Application Notes | For IF: Use at a dilution of 1:50-1:100. For WB: Use at a dilution of 1:100-1:200. For FACS: Use at an assay dependent dilution. This antibody is used in research to distinguish stratified epithelial cells from simple epithelial cells and identification of squamous cell carcinomas. The optimal conditions should be determined by the individual laboratory.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide |
