Application
IHC Image using - Detection of Cytokeratin 17 Human Squamous Cell Carcinoma| Product Name | Cytokeratin 17 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Cytokeratin 17This antibody is specific to 46 kD cytokeratin protein designated cytokeratin 17. It reacts with basal cells in complex epithelia and reacts strongly with squamous cell carcinomas. |
| Immunogen | Cytoskeletal fraction of rat colon epithelium. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | E3 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Gene Synonyms | 3872 KRT17 PCHC1 K17 Q04695 Cytokeratin 17 PC2 148069 PC Cytokeratin17 Ka17 |
|---|---|
| Target | Cytokeratin 17 |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | Kappa |
Application
IHC Image using - Detection of Cytokeratin 17 Human Squamous Cell Carcinoma| Positive Control | Skin |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
